הבעת חלבון refers to the synthesis of חלבונים within cells using information contained in the DNA or gene.
חלבונים are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study חלבון function in order to understand cellular processes.
This is currently studied based on using fluorescent חלבונים as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for חלבונים בעלי אופי ארעיים או ארעיים.
החוקרים דיווחו על טכניקה חדשה בשרת Preprint ב-30 ביולי 2020 שיכולה להתגבר על מגבלה זו.
The new study describes the use a fluorescent biosensor that enables real-time detection of חלבון expression in vivo which has implications for understanding the spatiotemporal expression of חלבונים within the living organism. This sensor is based on a dim green fluorescent חלבון in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a חלבון tag and enables detection of חלבון expression within seconds in live cells.
זֶה חיישן ביולוגי will be useful to study biological processes in real-time where חלבונים are either transiently expressed and/or to understand the expression and interactions of חלבונים from a disease-causing organism such as bacteria or a virus with that of the host proteins.
התייחסות:
Eason MG., Pandelieva AT., Mayer MM., et al 2020. ביוחיישן פלואורסצנטי מקודד גנטי לזיהוי מהיר של ביטוי חלבון. הדפסה מוקדמת: bioRxiv 2020.07.30.229633; DOI: https://doi.org/10.1101/2020.07.30.229633
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